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Image Search Results
Journal: Cancers
Article Title: DNAJA3/Tid1 Is Required for Mitochondrial DNA Maintenance and Regulates Migration and Invasion of Human Gastric Cancer Cells
doi: 10.3390/cancers12113463
Figure Lengend Snippet: Effect of Tid1 knockdown on cell migration and invasion of gastric cancer cells. ( A , B ) The effect of Tid1 knockdown on cell migration was evaluated by ( A ) the transwell migration assay and ( B ) the wound healing assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell migration was evaluated by the transwell assay (AGS for 8 h, NUGC-3 for 16 h, and TSGH9201 for 24 h) and the 24 h wound healing assay. ( C ) The effect of Tid1 knockdown on cell invasion was evaluated by the transwell invasion assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell invasion was evaluated by the transwell invasion assay for 48 h. The efficiency of Tid1 knockdown was confirmed by Western blot. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.
Article Snippet:
Techniques: Knockdown, Migration, Transwell Migration Assay, Wound Healing Assay, Transwell Assay, Transwell Invasion Assay, Western Blot, Control
Journal: Cancers
Article Title: DNAJA3/Tid1 Is Required for Mitochondrial DNA Maintenance and Regulates Migration and Invasion of Human Gastric Cancer Cells
doi: 10.3390/cancers12113463
Figure Lengend Snippet: Effect of Tid1 knockdown on mtDNA copy number and mitochondrial gene expression of gastric cancer cells. ( A ) The effect of Tid1 knockdown on mtDNA copy number was evaluated by real-time PCR. ( B – D ) The effect of Tid1 knockdown on mitochondrial gene expression was evaluated by reverse transcription (RT)-real-time PCR in the ( B ) AGS, ( C ) NUGC-3, and ( D ) TSGH 9201 gastric cancer cells. The efficiency of Tid1 knockdown was confirmed by Western blot or RT-real-time PCR. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.
Article Snippet:
Techniques: Knockdown, Gene Expression, Real-time Polymerase Chain Reaction, Reverse Transcription, Western Blot, Control