human gastric carcinoma cell line tsgh 9201 Search Results


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BioResource International Inc human gastric carcinoma cell line tsgh 9201
Human Gastric Carcinoma Cell Line Tsgh 9201, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tsgh 9201, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioResource International Inc gc cell lines tsgh 9201
Gc Cell Lines Tsgh 9201, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ags  (ATCC)
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ATCC ags
Ags, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Line Nugc 3, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioResource International Inc cell line ags
Effect of Tid1 knockdown on cell migration and invasion of gastric cancer cells. ( A , B ) The effect of Tid1 knockdown on cell migration was evaluated by ( A ) the transwell migration assay and ( B ) the wound healing assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell migration was evaluated by the transwell assay <t>(AGS</t> for 8 <t>h,</t> <t>NUGC-3</t> for 16 h, and TSGH9201 for 24 h) and the 24 h wound healing assay. ( C ) The effect of Tid1 knockdown on cell invasion was evaluated by the transwell invasion assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell invasion was evaluated by the transwell invasion assay for 48 h. The efficiency of Tid1 knockdown was confirmed by Western blot. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.
Cell Line Ags, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of Tid1 knockdown on cell migration and invasion of gastric cancer cells. ( A , B ) The effect of Tid1 knockdown on cell migration was evaluated by ( A ) the transwell migration assay and ( B ) the wound healing assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell migration was evaluated by the transwell assay (AGS for 8 h, NUGC-3 for 16 h, and TSGH9201 for 24 h) and the 24 h wound healing assay. ( C ) The effect of Tid1 knockdown on cell invasion was evaluated by the transwell invasion assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell invasion was evaluated by the transwell invasion assay for 48 h. The efficiency of Tid1 knockdown was confirmed by Western blot. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.

Journal: Cancers

Article Title: DNAJA3/Tid1 Is Required for Mitochondrial DNA Maintenance and Regulates Migration and Invasion of Human Gastric Cancer Cells

doi: 10.3390/cancers12113463

Figure Lengend Snippet: Effect of Tid1 knockdown on cell migration and invasion of gastric cancer cells. ( A , B ) The effect of Tid1 knockdown on cell migration was evaluated by ( A ) the transwell migration assay and ( B ) the wound healing assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell migration was evaluated by the transwell assay (AGS for 8 h, NUGC-3 for 16 h, and TSGH9201 for 24 h) and the 24 h wound healing assay. ( C ) The effect of Tid1 knockdown on cell invasion was evaluated by the transwell invasion assay. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and cell invasion was evaluated by the transwell invasion assay for 48 h. The efficiency of Tid1 knockdown was confirmed by Western blot. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.

Article Snippet: Human gastric cancer cells AGS, NUGC-3, and TSGH-9201 were purchased from the Bioresource Collection and Research Center (Food Industry Research and Development Institute, Hsinchu, Taiwan) and cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% antibiotic (penicillin-streptomycin, Biological Industries, Kibbutz Beit HaEmek, Israel), and incubated at 37 °C in a 5% CO 2 -containing incubator.

Techniques: Knockdown, Migration, Transwell Migration Assay, Wound Healing Assay, Transwell Assay, Transwell Invasion Assay, Western Blot, Control

Effect of Tid1 knockdown on mtDNA copy number and mitochondrial gene expression of gastric cancer cells. ( A ) The effect of Tid1 knockdown on mtDNA copy number was evaluated by real-time PCR. ( B – D ) The effect of Tid1 knockdown on mitochondrial gene expression was evaluated by reverse transcription (RT)-real-time PCR in the ( B ) AGS, ( C ) NUGC-3, and ( D ) TSGH 9201 gastric cancer cells. The efficiency of Tid1 knockdown was confirmed by Western blot or RT-real-time PCR. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.

Journal: Cancers

Article Title: DNAJA3/Tid1 Is Required for Mitochondrial DNA Maintenance and Regulates Migration and Invasion of Human Gastric Cancer Cells

doi: 10.3390/cancers12113463

Figure Lengend Snippet: Effect of Tid1 knockdown on mtDNA copy number and mitochondrial gene expression of gastric cancer cells. ( A ) The effect of Tid1 knockdown on mtDNA copy number was evaluated by real-time PCR. ( B – D ) The effect of Tid1 knockdown on mitochondrial gene expression was evaluated by reverse transcription (RT)-real-time PCR in the ( B ) AGS, ( C ) NUGC-3, and ( D ) TSGH 9201 gastric cancer cells. The efficiency of Tid1 knockdown was confirmed by Western blot or RT-real-time PCR. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.

Article Snippet: Human gastric cancer cells AGS, NUGC-3, and TSGH-9201 were purchased from the Bioresource Collection and Research Center (Food Industry Research and Development Institute, Hsinchu, Taiwan) and cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% antibiotic (penicillin-streptomycin, Biological Industries, Kibbutz Beit HaEmek, Israel), and incubated at 37 °C in a 5% CO 2 -containing incubator.

Techniques: Knockdown, Gene Expression, Real-time Polymerase Chain Reaction, Reverse Transcription, Western Blot, Control